Journal: PLoS ONE

Article Title: Poxvirus-Based Active Immunotherapy with PD-1 and LAG-3 Dual Immune Checkpoint Inhibition Overcomes Compensatory Immune Regulation, Yielding Complete Tumor Regression in Mice

doi: 10.1371/journal.pone.0150084

Figure Lengend Snippet: CT26-HER-2 cells were stimulated with varying concentrations of IFNγ for 18 hours. A) Percent of cells expressing PD-L1 and the mean fluorescence intensity (MFI) by flow cytometry. B) Cells were stimulated with IFNγ for 18 hours at concentrations indicated in each panel then stained for PD-L1 (red) and a nuclei stain (DAPI, blue).

Article Snippet: The CT26-HER-2 and MC38-MUC1 tumor cell lines were treated with recombinant mouse IFNγ at indicated concentrations for 18 hours (0–1000 ng/mL, Millipore, Temecula, CA).

Techniques: Expressing, Fluorescence, Flow Cytometry, Staining

Journal: Oncotarget

Article Title: Trans -scirpusin A showed antitumor effects via autophagy activation and apoptosis induction of colorectal cancer cells

doi: 10.18632/oncotarget.17388

Figure Lengend Snippet: (A) Specific lysis of hp63 (TYLPTNASL) peptide-loaded target cells was estimated by in vivo CTL (Student's t - t est; n=5 per group). (B) mRNA was isolated from the tumors and the mRNA levels of TNF-α were analyzed by RT-PCR (*p<0.05., Student's t -test; n=5 per group). (C) Her2-specific Abs were assessed from the sera of Her2/CT26 tumor-bearing mice treated with TSA or vehicle. Her2/CT26 cells were stained with each sera, followed by staining with FITC-conjugated secondary anti-IgG Abs (Student's t -test; n=5 per group).

Article Snippet: Briefly, Her2/CT26 cells were stained with diluted sera containing anti-Her2 polyclonal Abs, and then FITC-labeled goat anti-mouse IgG Abs (BD Bioscience, San Diego, CA) were added to detect cell-bound murine IgG.

Techniques: Lysis, In Vivo, Isolation, Reverse Transcription Polymerase Chain Reaction, Staining